Axonal and dendritic synaptotagmin isoforms revealed by a pHluorin-syt functional screen
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چکیده
منابع مشابه
Axonal and dendritic synaptotagmin isoforms revealed by a pHluorin-syt functional screen
The synaptotagmins (syts) are a family of molecules that regulate membrane fusion. There are 17 mammalian syt isoforms, most of which are expressed in the brain. However, little is known regarding the subcellular location and function of the majority of these syts in neurons, largely due to a lack of isoform-specific antibodies. Here we generated pHluorin-syt constructs harboring a luminal doma...
متن کاملCharacterization of KIAA1427 protein as an atypical synaptotagmin (Syt XIII).
Synaptotagmin (Syt) belongs to a family of type-I membrane proteins and is a protein that consists of a short extracellular N-terminus, a single transmembrane domain, two C2 domains and a short C-terminus. Here, we cloned and characterized a mouse orthologue of human KIAA1427 protein as an atypical Syt (named Syt XIII). Subcellular fractionation and antibody-uptake experiments indicate that Syt...
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Regulated exocytosis in chromaffin cells is triggered by membrane depolarization and subsequent Ca influx through voltage-gated channels. The level of Ca accumulation is commensurate with the strength of stimulation (Douglas and Rubin, 1961; Neher and Augustine, 1992; Fulop and Smith, 2007; de Diego et al., 2008). Ca drives exocytosis through the Ca-binding synaptotagmin (Syt) protein family (B...
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Synaptotagmin (Syt) is involved in Ca2+ -regulated secretion and has been suggested to serve as a general Ca2+ sensor on the membrane of secretory vesicles in neuronal cells. Insulin exocytosis from the pancreatic beta-cell is an example of a Ca2+ -dependent secretory process. Previous studies have yielded conflicting results as to which Syt isoform is present on the secretory granules in the n...
متن کاملSynaptotagmin (Syt) IX is an essential determinant for protein sorting to secretory granules in mast cells.
The secretory granules (SGs) of secretory cells of the hematopoietic lineage, such as the mast cells, are lysosome-related organelles whose membrane proteins travel through the plasma membrane and the endocytic system. Therefore, a mechanism must exist to prevent proteins destined to recycling or to the trans-Golgi network (TGN) from reaching the SGs. We now show that synaptotagmin (Syt) IX, a ...
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ژورنال
عنوان ژورنال: Molecular Biology of the Cell
سال: 2012
ISSN: 1059-1524,1939-4586
DOI: 10.1091/mbc.e11-08-0707